"HEK293 cells were cultured at a density of 3.5 × 105 cells/mL in DMEM supplemented with 10% FBS, L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin overnight. The cells were transfected with each plasmid (pcDNA3.1-SARS2-Spike, pcDNA3-SARS-CoV-2-S-RBD-sfGFP, or pcDNA3.1-hACE2) and incubated for 22 h. After incubation, the cultured cells were scraped and washed with ice-cold Dulbecco’s phosphate-buffered saline (D-PBS). Cell counting was performed and xTractor buffer (Takara Bio Inc., Shiga, Japan) was added to the cell precipitate. The cell lysates were centrifuged at 1300× g for 10 min at 4 °C and the supernatant was transferred to new tubes and stored at −80 °C until use. The protein concentration was determined by bicinchoninic acid (BCA) protein assay using a BCA assay kit (Takara). Ten microliters of nattokinase and 10 µL of cell lysate (1 μ µg/mL) were incubated at 37 °C for 1 h. When the effects of protease inhibitors were used, protease inhibitor cocktail sets I and III were diluted 10-fold with D-PBS, and a 10 µL protease inhibitor cocktail solution was added to the mixture of nattokinase and cell lysate. Equal volumes of the reaction mixture were loaded and Western blotting was performed. The primary antibodies included anti-rhodopsin (C9) mouse monoclonal antibody (1D4) (Santa Cruz Biotechnology, Dallas, TX, USA), anti-GAPDH mouse monoclonal antibody (FUJIFILM Wako), anti-GFP tag mouse monoclonal antibody (Proteintech, Rosemont, IL, USA), and anti-ACE2 antibody (Proteintech). Secondary antibodies include HRP-conjugated goat anti-mouse antibody (Proteintech)." https://www.mdpi.com/1420-3049/27/17/5405
Confidently incorrect again folks. I'm suggesting you get your medical advice from medical experts, not anonymous people on social media.
Especially not from anonymous loose cannons like the V&C1 committee, Check out their post history - you can tell when they change shifts and the new one sits down at the keyboard.
Sounds real handy.
"HEK293 cells were cultured at a density of 3.5 × 105 cells/mL in DMEM supplemented with 10% FBS, L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin overnight. The cells were transfected with each plasmid (pcDNA3.1-SARS2-Spike, pcDNA3-SARS-CoV-2-S-RBD-sfGFP, or pcDNA3.1-hACE2) and incubated for 22 h. After incubation, the cultured cells were scraped and washed with ice-cold Dulbecco’s phosphate-buffered saline (D-PBS). Cell counting was performed and xTractor buffer (Takara Bio Inc., Shiga, Japan) was added to the cell precipitate. The cell lysates were centrifuged at 1300× g for 10 min at 4 °C and the supernatant was transferred to new tubes and stored at −80 °C until use. The protein concentration was determined by bicinchoninic acid (BCA) protein assay using a BCA assay kit (Takara). Ten microliters of nattokinase and 10 µL of cell lysate (1 μ µg/mL) were incubated at 37 °C for 1 h. When the effects of protease inhibitors were used, protease inhibitor cocktail sets I and III were diluted 10-fold with D-PBS, and a 10 µL protease inhibitor cocktail solution was added to the mixture of nattokinase and cell lysate. Equal volumes of the reaction mixture were loaded and Western blotting was performed. The primary antibodies included anti-rhodopsin (C9) mouse monoclonal antibody (1D4) (Santa Cruz Biotechnology, Dallas, TX, USA), anti-GAPDH mouse monoclonal antibody (FUJIFILM Wako), anti-GFP tag mouse monoclonal antibody (Proteintech, Rosemont, IL, USA), and anti-ACE2 antibody (Proteintech). Secondary antibodies include HRP-conjugated goat anti-mouse antibody (Proteintech)." https://www.mdpi.com/1420-3049/27/17/5405
Your opinion as a fat, triple vaxed, contagious, uneducated mechanic is noted.
As an obese boomer, you are scientifically proven to be a Covid Super Spreader.
I wonder if she snorts her nattokinase or injects it folks.
Or just laps it up straight out of the petri dish.
Remember folks Tuchodi is a fat boomer and uneducated mechanic telling strangers to inject an experimental drug with no long term data
Confidently incorrect again folks. I'm suggesting you get your medical advice from medical experts, not anonymous people on social media.
Especially not from anonymous loose cannons like the V&C1 committee, Check out their post history - you can tell when they change shifts and the new one sits down at the keyboard.